Abstract
The micropropagation system described is a commercially viable method for asexual propagation of a mature ‘elite’ tree of Acacia confusa. The main stem of a mature tree was hardly pruned to induce new shoots for the explants. This study showed that PPM™ (Plant Preservative Mixture) was an effective agent for controlling the contamination of nodal explants and was added to the initial culture medium. This medium consisted of Murashige and Skoog (MS) basal salts and vitamins, supplemented with 4.0 mg L−1 BA, 0.1 mg L−1 IBA, 10 mg L−1 GA, 12 mg L−1 AS, 3.2 g L−1 PVP, and 2.0 mL L−1 PPM™, and was adequate for the establishment of in vitro mother stock for proliferation. The excellent growth response of nodal explants, obtained from May to July in 2019 to 2020, had shoot formation rate ranging from 15.8 to 18.5%. The optimal shoot multiplication was derived on MS medium supplemented with 1.0 mg L−1 BA, 0.5 mg L−1 TDZ, 12 mg L−1 AS, and 500 mg L−1 glutamine which could support feasible 23.7 ± 3.4 shoot proliferation per explant. Shoots generated in vitro with a length of 3 to 5 cm were excised and cultured on MS with 1.0 mg L−1 IBA for rooting. The rooting frequency could be increased to 72.2% within 6 wk. Rooted plantlets in vitro were acclimatized for 4 wk before transplanting to the pots in a greenhouse.
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References
Banerjee P (2013) Rapid in vitro propagation of Acacia auriculiformis on solid and liquid media: role of organic additive, antioxidant and plant growth regulators. Cibtech J Bio Protocols 2:39–49
Beck SL, Dunlop RW (2001) Micropropagation of the Acacia species – a review. In Vitro Cell Dev Biol - Plant 37:531–538
Bonga JM, Klimaszewska KK, von Aderkas P (2010) Recalcitrance in clonal propagation, in particular of conifers. Plant Cell Tiss Org Cult 100:241–254
Chamandoosti F (2010) The relationship between plant growth regulators for organogenesis and phenolic compound in cotton (Gossypium hirsutum L.). Asian J Dev Biol 2:16–22
Chang SH, Ho CK, Tsay JY (2002) In vitro culture of Cinnamomum kanehirae Hay. Taiwan J for Sci 17:491–501 ([in Chinese with English summary])
Chung JP, Chen KH (2018) Developing an in vitro quasi-symbiotic culture system of Aquilaria malaccensis with potentially beneficial microbes on agarwood resinous formation. Plant Cell Tiss Org Cult 133:193–202
Compton ME, Koch JM (2001) Influence of plant preservative mixture (PPMTM) on adventitious organogenesis in melon, petunia, and tobacco. In Vitro Cell Dev Biol - Plant 37:259–261
Darus HA (1990) Micropropagation of Acacia mangium from aseptically germinated seedlings. J Trop for Sci 3:204–208
Gantait S, Kundu S, Das PK (2018) Acacia: an exclusive survey on in vitro propagation. J Saudi Soc Agric Sci 17:163–177
Girijashankar V (2011) Micropropagation of multipurpose medicinal tree Acacia auriculiformis. J Med Plants Res 5:462–466
Greenwell Z, Ruter J (2018) Effect of glutamine and arginine on growth of Hibiscus moscheutos in vitro. Ornam Hortic 24(4):393–399
Hackett WP (2011) Juvenility, maturation, and rejuvenation in woody plants. John Wiley & Sons, Ltd
Hartman HT, Kester DE, Davies FT Jr, Geneve RL (2014) Hartmann & Kester’s plant propagation: principles and practices, 8th edn. Pearson Education Inc, Upper Saddle River
Huang FH, Al-Khayri JM, Gbur EE (1994) Micropropagation of Acacia mearnsii. In Vitro Cell Dev Biol - Plant 30:70–74
Huang LJ, Chen ZX, Zhang SQ, Liang RG (2012) Tissue culture technique of Acacia mangium elite trees. For Res 25:227–230
Huetteman CA, Preece JE (1993) TDZ: a potent cytokinin for woody plant tissue. Plant Cell Tiss Org Cult 33:105–119
Lloyd G, McCown B (1980) Commercially feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot tip culture. Comb Proc Int Plant Prop Soc 30:421–427
Kartsonas E, Papafotiou M (2007) Mother plant age and seasonal influence on in vitro propagation of Quercus euboica Pap., an endemic, rare and endangered oak species of Greece. Plant Cell Tiss Org Cult 90:111–116
Ko W, Su C, Chen C, Chao C (2009) Control of lethal browning of tissue culture plantlets of Cavendish banana cv. formosana with ascorbic acid. Plant Cell Tiss Org Cult 96:141–145
Mathur I, Chandra N (1983) Induced regeneration in stem explants of Acacia nilotica. Curr Sci 52:882–883
McCown BH (2000) Woody shrubs and trees. In: Smith RH (ed) Plant tissue culture techniques and experiments 2nd. Academic Press, New York, pp 123–134
Mittal A, Agrawal R, Gupta SC (1989) In vitro development of plantlets from axillary buds of Acacia auriculiformis. Plant Cell Tiss Org Cult 19:65–70
Miyazaki J, Tan BH, Errington SG (2010) Eradication of endophytic bacteria via treatment for axillary buds of Petunia hybrida using Plant Preservative Mixture (PPMTM). Plant Cell Tiss Org Cult 102:365–372
Monteuuis O, Galiana A, Goh D (2013) In vitro propagation of Acacia mangium, and A. mangium x A. auriculiformis. In: Lambardi M, Ozudogru EA, Jain SM (eds) Protocols for micropropagation of selected economically-important horticultural plants. Springer, New York, pp 199–211
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Nandwani D (1995) In vitro micropropagation of a tree legume adapted to arid lands: Acacia tortilis subsp. radiana. Ann for Sci 52:183–189. https://doi.org/10.1051/forest:19950208
Nangia S, Singh R (1996) Micropropagation of Acacia tortilis Hayne (Umbrella thorn) through cotyledonary node culture. Indian J Exp Physiol 1:77–79
Ogita S, Sasamoto H, Yeung EC, Thorpe TA (2001) The effects of glutamine of the maintenance of embryogenic cultures of Cryptomeria japonica. In Vitro Cell Dev Biol - Plant 37:268–273
Palma B, Vogt GF, Neville P (1996) A combined in vitro/in vivo method for improved grafting of Acacia senegal (L.) Willd. Hort Sci 3:379–381
Palmberg C (1981) A vital fuelwood gene pool is in danger. Unasylva 33:22–30
Quoirin M (2003) Micropropagation of Acacia species. In: Jain SM, Ishii K (eds) Micropropagation of woody trees and fruits. Forestry Sciences, vol 75. Springer, Dordrecht, pp 245–268. https://doi.org/10.1007/978-94-010-0125-0_9
Reustle G, Natter I (1994) Effect of polyvinylpyrrolidone and activated charcoal on formation of microcallus from grapevine protoplasts (Vitis sp.). Vitis 33:117–121
Rihan HZ, Al-Issawi M, Al-swedi F, Fuller MP (2012) The effect of using PPM (plant preservative mixture) on the development of cauliflower microshoots and the quality of artificial seed produced. Sci Hort 141:47–52
Rout GR, Senapati SK, Aparajeta S (2008) Micropropagation of Acacia chundra (Roxb.) DC. Hort Sci 35:22–26. https://doi.org/10.17221/648-HORTSCI
Saxena PK, Gill R (1986) Removal of browning and growth enhancement by polyvinylpolypyrrolidone in protoplast cultures of Cyamopsis tetragonoloba L. Biol Plant 28:313–315
Simmons MH (1987) The genus Acacia. In: Simmons MH (ed) Acacias of Australia, vol 1. Nelson, South Melbourne, pp 7–10
Singh HP, Singh S, Saxena RP, Singh RK (1993) In vitro bud break in axillary nodal segments of mature trees of Acacia nilotica. Indian J Plant Physiol 36:21–24
Skolmen RG (1986) Acacia (Acacia koa Gray). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 1. trees 1. Springer-Verlag, Berlin, pp 3755–4384
Skolmen RG, Mapes MO (1976) Acacia koa Gray plantlets from somatic callus tissue. J Hered 67:114–115
Souza G, Lone A, Faria R, Oliveira K (2013) Pulp fruit added to culture medium for in vitro orchid development. Semin Cienc Agrar 34:1141–1146
Thomas TD (2008) The role of activated charcoal in plant tissue culture. Biotechnol Adv 26:618–631
Vengadesan G, Ganapathi A, Anand RP, Anbazhagan VR (2000) In vitro organogenesis and plant formation in Acacia sinuata (Lour.) Merr. Plant Cell Tiss Org Cult 6:23–28
Vengadesan G, Ganapathi A, Anand RP, Selvaraj N (2003) In vitro propagation of Acacia sinuata (Lour.) Merr. from nodal segments of a 10- year-old tree. In Vitro Cell Dev Biol - Plant 39:409–414
Vengadesan G, Ganapathi A, Amutha S, Selvaraj N (2002) In vitro propagation of Acacia species-a review. Plant Sci 163:663–671
Xie D, Hong Y (2001a) In vitro regeneration of Acacia mangium via organogenesis. Plant Cell Tiss Org Cult 66:167–173
Xie D, Hong Y (2001b) Regeneration of Acacia mangium through somatic embryogenesis. Plant Cell Rep 20:34–40
Zhang HW, Huand XL, Fu JR, Yang MQ, Chen CQ (1995) Axillary bud culture and plantlet regeneration of Acacia auriculiformis and A. mangium. J Trop Subtrop Bot 3:62–68
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This research received financial support from the Forestry Bureau, Council of Agriculture, Executive Yuan, Taiwan (ROC) under Contract No. tfbc-1080504.
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Ho, WJ., Huang, YK., Huang, WW. et al. Effective in vitro culture using dormant bud of nodal sections from a mature Acacia tree. In Vitro Cell.Dev.Biol.-Plant 58, 437–446 (2022). https://doi.org/10.1007/s11627-021-10235-8
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DOI: https://doi.org/10.1007/s11627-021-10235-8