Skip to main content
SpringerLink
Log in

Shoot organogenesis and plant regeneration in Cordia subcordata Lam

  • Micropropagation
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

Cordia subcordata Lam. (Boraginaceae) is a woody tree that grows in coastal wasteland and mangrove edges. Its wood is used for woodcraft, and the tree, which is used in landscaping, also has medicinal and ecological value (e.g., coastal protection). In this study, a shoot proliferation and regeneration system was established for this plant. Callus was induced from terminal young leaf explants on Murashige and Skoog (MS) medium with 1.0 mg L–1 thidiazuron or 1.0 mg L–1 2,4-dichlorophenoxyacetic acid, or optimally with 0.5 or 1.0 mg L–1 of both. Callus differentiated from an average of 35.4% of explants into 3 to 5 adventitious shoots (indirect shoot organogenesis) within 45 d. Optimal shoot proliferation medium was MS medium with 0.8 mg L–1 6-benzyladenine (BA). A higher concentration of BA (1.5 mg L–1) in the presence of α-naphthaleneacetic acid (NAA) enhanced callus induction from the base of shoots and decreased shoot proliferation. On ½MS medium with 0.5 mg L–1 NAA and/or indole-3-butyric acid, adventitious roots were induced from shoots. Within 1 mo, 95.4% of plantlets transferred to a substrate of vermiculite and peat (1:1, v/v) survived.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.

Similar content being viewed by others

Data availability

All data generated or analyzed during this study are included in this published article.

References

  • Abbott IA, Shimazu C (1985) The geographic origin of the plants most commonly used for medicine by Hawaiians. J Ethnopharmacol 14:213–222

    Article  CAS  Google Scholar 

  • Allen JA (2002) Cordia subcordata Lam. In: Vozzo J (ed) Tropical tree seed manual: part II, species descriptions, Agricultural handbook 712. US Department of Agriculture, Washington DC, pp 418–419

    Google Scholar 

  • Boshier DH, Chase MR, Bawa KS (1995) Population genetics of Cordia alliodora (Boraginaceae), a Neotropical tree 2. Mating System Amer J Bot 82:476–483

    Article  Google Scholar 

  • Canché-Collí C, Canto A (2014) Distylous traits in Cordia dodecandra and Cordia sebestena (Boraginaceae) from the Yucatan Peninsula. Bot Sci 92:289–297

    Article  Google Scholar 

  • Dewir YH, Nurman S, Naidoo Y, Teixeira da Silva JA (2018) Thidiazuron-induced abnormalities in plant tissue cultures. Plant Cell Rep 37:1451–1470

    Article  CAS  Google Scholar 

  • Friday JB, Okano D (2006) Cordia subcordata (kou). In: Species profiles for Pacific Island agroforestry, 15 pp. https://agroforestry.org/images/pdfs/Cordia-kou.pdf. Accessed 6 Oct 2021

  • Gandhimathi R, Kumar AS (2009) Evaluation of antioxidant activity of Cordia subcordata Lam. against carbon tetrachloride (CCl4) induced erythrocyte damage in rats. Pharmacol Online 2:720–727

    Google Scholar 

  • Jayasuriya A, Gunatilleke, (2015) A refugium for Cordia subcordata (Boraginaceae), a very rare and endangered plant in Sri Lanka and strategies for its conservation. Ceylon J Sci 44:67–70

    Google Scholar 

  • Krishna H, Singh D (2013) Micropropagation of lasora (Cordia myxa Roxb.). Indian J Hortic 70:323–327

    Google Scholar 

  • Kumar SA, Gandhimathi R, Senthil K, Kumar KP (2009) Hepatoprotective potential of Cordia subcordata Lam. against carbon tetra chloride (CCl4)-induced hepatotoxicity in Wistar albino rats. J Biomed Sci Res 1:19–26

    Google Scholar 

  • Lameira OA, Pinto J (2006) In vitro propagation of Cordia verbenacea L. (Boraginaceae). Rev Bras Plantas Med 8:102–104 (in Portuguese with English abstract)

    Google Scholar 

  • Liu DM, Chen HF, Wang FG, Xing FW (2015) Investigation of introduced plants in Nansha Islands and reefs, China. J Trop Subtrop Bot 23:167–175 (in Chinese with English abstract)

    Google Scholar 

  • Liu DM, Jian SG, Ren H, Chen HF, Xing FW (2017) A method of cutting in Cordia subcordata. Patent: 201710110479.8 (in Chinese)

  • Lloyd G, McCown BH (1980) Commercially-feasible micropropagation of mountain laurel (Kalmia latifolia) by shoot tip culture. Proc Intl Plant Prop Soc 30:421–427

    Google Scholar 

  • Londono-Giraldo LM, Gutierrez-Lopez LG (2013) Regeneración de nogal cafetero (Cordia alliodora (Ruizy pav.) Oken, a partir de organogénesis indirecta. Acta Agron 62:207–214 (in Spanish with English abstract)

    Google Scholar 

  • Mantovani NC, Franco E, Silvane V (2001) In vitro regeneration of louro-pardo (Cordia trichotoma (Vellozo) Arrabida ex Steudel). Ciência Florestal 11:93–101 (in Portuguese with English abstract)

    Article  Google Scholar 

  • Matias EFF, Alves EF, Silva MK do N, Carvalho VRDA, Costa JGMD (2015) The genus Cordia: botanists, ethno, chemical and pharmacological aspects. Rev Bras Farmacog 25:542–552 (in Portuguese with English abstract)

  • Miller JS (1988) Systematics of the genus Cordia (Boraginaceae) in Mexico and Central America. PhD thesis, St. Louis University, St. Louis, MO, USA. http://aims.fao.org/aos/agrovoc/c_27165. Accessed 6 Oct 2021

  • Murashige T, Skoog F (1962) A revised medium for medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Santacruz Ruvalcaba F, Castañeda Nava JJ, Gaspar Peralta AM, Núñez Sandoval N, Mora Santacruz A (2014) Breaking of dormancy in seeds and in vitro propagation of Cordia elaeagnoides A. DC Rev Mex Ciencias for 5:84–97 (in Spanish with English abstract)

    Google Scholar 

  • Schuler GI, Baquero SO, Gaona DT, Vega EG, Rodríguez JR, Ramírez CS, Nieto VR, de Jaramillo EH (2005) In vitro propagation of Tabebuia rosea (Bertol. DC) (large spreading cedar) and Cordia alliodora (Ruiz & Pav.) Oken (coffee-shading walnut). Rev Colomb Biotecnol 7:39–50 (in Spanish with English abstract)

    Google Scholar 

  • Taha A (2016) Callus induction and plant regeneration of Cordia myxa via tissue culture system. Int J Pharma Biosci 4:18–29

    Google Scholar 

  • Thaman RR (1994) Ethnobotany of Pacific Island coastal plants. Sci Pacific Island Peoples 3:147–184

    Google Scholar 

  • Wang XP, Wen MH, Wu MS, Zhang DX (2020) Cordia subcordata (Boraginaceae), a distylous species on oceanic coral islands, is self-compatible and pollinated by a passerine bird. Plant Ecol Evol 153:361–372

    Article  Google Scholar 

  • Whistler WA (1992) Polynesian herbal medicine. Everbest, Hong Kong, pp 138–139

    Google Scholar 

  • Wu SH, Chen HW, Jian SG, Liu H, Zhang W, Ren H (2017) The biological characteristics of Cordia subcordata on tropical coral island in China. Ecol Sci 36:57–63 (in Chinese with English abstract)

    Google Scholar 

  • Xiong YP, Liang HZ, Yan HF, Guo BY, Niu MY, Chen SY, Jian SG, Ren H, Zhang XH, Li Y, Zeng SJ, Wu KL, Zheng F, Teixeira da Silva JA, Ma GH (2019) NaCl-induced stress: physiological responses of six halophyte species in in vitro and in vivo culture. Plant Cell Tiss Org Cult 139:531–546

    Article  CAS  Google Scholar 

  • Yang XY, Lü JF, Teixeira da Silva JA, Ma GH (2012) Somatic embryogenesis and shoot organogenesis from leaf explants of Primulina tabacum. Plant Cell Tiss Org Cult 109:213–222

    Article  Google Scholar 

Download references

Funding

This work was financially supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA13020500) and the National Science and Technology Support Program (2015BAL04B04).

Author information

Author notes

  1. Yuping Xiong and Xiaohong Chen contributed equally to this work.

Authors and Affiliations

  1. Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou, 510650, China

    Yuping Xiong, Xiaohong Chen, Kunlin Wu, Songjun Zeng & Guohua Ma

  2. Ikenobe, Japan

    Jaime A. Teixeira da Silva

Authors
  1. Yuping Xiong
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaohong Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kunlin Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jaime A. Teixeira da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Songjun Zeng
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Guohua Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

YPX and GHM designed the experiment and provided guidance for the study. YPX and XHC prepared samples for all analyses. KLW, SJZ, and GHM conducted the statistical analyses. JATS provided advice, interpretation of the experiment and data, and co-wrote the manuscript with GHM. All the authors read and approved the manuscript for publication.

Corresponding author

Correspondence to Guohua Ma.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

Not applicable.

Competing interests

The authors declare no competing interests.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xiong, Y., Chen, X., Wu, K. et al. Shoot organogenesis and plant regeneration in Cordia subcordata Lam. In Vitro Cell.Dev.Biol.-Plant 58, 392–398 (2022). https://doi.org/10.1007/s11627-021-10233-w

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-021-10233-w

Keywords

Search

Navigation