Skip to main content
SpringerLink
Log in

Better light spectral quality and thermal amplitude inside the greenhouse stimulate growth and improve acclimatization of in vitro–grown Cattleya warneri T. Moore

  • Plant Tissue Culture
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

Growth and photosynthetic capacity of Cattleya warneri T. Moore seedlings cultivated in vitro were evaluated in two environments: (1) growth room (GR) with constant light, humidity, and temperature; and (2) greenhouse (GH) with variable humidity, temperature, and light intensity and quality. In both environments, two different tissue culture vessel lids were used: transparent plastic lids (TCVplastic) and non-transparent metal lids (TCVmetal). After 11 months of in vitro cultivation, five seedlings from each tissue culture vessel were evaluated for growth and photosynthetic capacity, while the other five seedlings of each tissue culture vessel were transferred to a greenhouse for acclimatization. Increased biomass production in vitro was observed in GH and GR (GH>GR). However, the photosynthetic capacity was not altered by the GH environment, since the net photosynthetic rate at 300 μmol m−2 s−1 (NPR300) was low in the final of period in all treatments. Therefore, the increase in biomass production in C. warneri was mostly dependent on the exogenous carbon source through the addition of sucrose to the culture medium. The use of TCVplastic in vitro improved seedlings’ growth in both GR and GH, showing an advantage in relation to TCVmetal. GH environment with quality light spectrum and thermal amplitude, and use of TCVplastic, increased biomass production in vitro and improved the acclimatization process of C. warneri seedlings, which also reduced electricity costs since the use of artificial light and air conditioning is not required.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.
Figure 3.
Figure 4.
Figure 5.
Figure 6.
Figure 7.
Figure 8.

Similar content being viewed by others

Abbreviations

Fv/Fm :

Maximum quantum yield of photosystem II;

GH:

Greenhouse;

GR:

Growth room;

IVH:

In vitro hardening;

PAR:

Photosynthetically active radiation;

DLI:

Daily light integral;

PI:

Photosynthetic index;

RDW:

Root dry weight;

RFW:

Root fresh weight;

RH:

Relative humidity;

SDW:

Shoot dry weight;

SFW:

Shoot fresh weight;

Tair:

Air temperature;

TCVmetal :

Tissue culture vessel with non-transparent metal lids;

TCVplastic :

Tissue culture vessel with transparent plastic lids;

TCV:

Tissue culture vessel;

TDW:

Total dry weight;

TFW:

Total fresh weight;

VPDair :

Air vapor pressure deficit 

References

  • Abreu PP, Souza MM, Almeida AAF, Santos EA, Freitas JCO, Figueiredo AL (2014) Photosynthetic responses of ornamental passion flower hybrids to varying light intensities. Acta Physiol Plant 36:1993–2004

  • Batista DS, Felipe SHS, Silva TD, De Castro KM, Mamedes-Rodrigues TC, Miranda NA, Ríos-Ríos AM, Faria DV, Fortini EA, Chagas K, Torres-Silva G, Xavier A, Arencibia AD, Otoni WC (2018) Light quality in plant tissue culture: does it matter? In Vitro Cell Dev Biol - Plant 54:195–215

    Article  CAS  Google Scholar 

  • Bennett SM, Tibbitts TW, Cao W (1991) Diurnal temperature fluctuation effects on potatoes grown with 12 h photoperiod. Am Potato J 68:81–86

    Article  CAS  PubMed  Google Scholar 

  • Bohlar-Nordenkampf HR, Draxler G (1993) Functional leaf anatomy. In: Hall DO, Scurlock JMO, Bohlar-Nordenkampf HR, Leegood LC, Long SP (eds) Photosynthesis and productioan in a changing environmental: a field and laboratory manual. Chapman & Hall, London, pp 91–112

    Google Scholar 

  • Borges D, Oliveira MC, Penoni ES, Pádua TRP, Braga FT, Pasqual M (2011) Microprogation of chrysanthemum (Dendranthema grandiflora tzevele cv. rage) under natural and artificial light in different concentration of the culture media. Plant Cell Cult Microprop 7:1–8

    Google Scholar 

  • Cassells AC, Walsh C (1994) The influence of gas permeability of the culture lid on calcium uptake and stomatal function in Dianthus microplants. Plant Cell Tiss Org Cult 37:171–178

    Article  Google Scholar 

  • Cerasoli S, Wertin T, Mcguire MA, Rodrigues A, Aubrey DP, Pereira JS, Teskey RO (2014) Poplar saplings exposed to recurring temperature shifts of different amplitude exhibit differences in leaf gas exchange and growth despite equal mean temperature. AoBP 6:1–9

    Google Scholar 

  • Chen C (2006) In situ measurement of microclimate for the plantlets cultured in vitro. Biosyst Eng 95:413–423

    Article  Google Scholar 

  • De Riek J, Piqueras A, Deberegh PC (1997) Sucrose uptake and metabolism in a double layer system for micropropagation of Rosa multiflora. Plant Cell Tiss Org Cult 47:269–278

    Article  Google Scholar 

  • Demmig B, Björkman O (1987) Comparison of the effect of excessive light on chlorophyll fluorescence (77K) and photon yield of O2 evolution in leaves of higher plants. Planta 171:171–184

  • Deng R, Donnelly DJ (1993) In vitro hardening of red raspberry through CO2 enrichment and relative humidity reduction on sugar-free medium. Can J Plant Sci 73:1105–1113

    Article  CAS  Google Scholar 

  • Erig AC, Schuch MW (2005) Micropropagação fotoautotrófica e uso da luz natural. Cienc Rural 35:961–965

    Article  Google Scholar 

  • Ferreira WM, Suzuki RM (2008) O cultivo in vitro de orquídeas como alternativa para a preservação de espécies nativas ameaçadas de extinção. In: Loiola MIB, Baseia IG, Lichston JE (eds) Atualidades, desafios e perspectiva da botânica no Brasil. Imagem Gráfica, Natal, pp 67–68

    Google Scholar 

  • Fini A, Loreto F, Tattini M, Giordano C, Ferrini F, Brunetti C, Cetritto M (2016) Mesophyll conductance plays a central role in leaf functioning of Oleaceae species exposed to contrasting sunlight irradiance. Physiol Plant 157:54–68

    Article  CAS  PubMed  Google Scholar 

  • Fuentes G, Talavera C, Desjardins Y, Santamaría JM (2007) Low exogenous sucrose improves ex vitro growth and photosynthesis in coconut in vitro plantlets if grown in vitro under high light. Acta Hort 748:151–156

    Article  CAS  Google Scholar 

  • Fujiwara K, Kozai T (1995) Physical microenvironment and its effects. In: Aitken-Christie J, Kozai T, Smith MAL (eds) Automation and environmental control in plant tissue culture. Springer, Dordrecht, pp 319–369

    Chapter  Google Scholar 

  • Genty B, Goulas Y, Dimon B, Peltier JM, Moya I (1992) Modulation of efficiency of primary conversion in leaves, mechanisms involved at PSII. In: Murata N (ed) Research in Photosynthesis. Volume 4. Dordrecht, Kluwer Academic Publishers, pp 603–10

  • Gilliham M, Dayod M, Hocking BJ, Xu B, Conn SJ, Kaiser BN, Leigh RA, Tyerman SD (2011) Calcium delivery and storage in plant leaves: exploring the link with water flow. J Exp Bot 62:2233–2250

    Article  CAS  PubMed  Google Scholar 

  • Hdider C, Desjardins Y (1994) Effects of sucrose on photosynthesis and phosphoenol pyruvate carboxylase activity of in vitro cultured strawberry plantlets. Plant Cell Tiss Org Cult 36:27–36

    Article  CAS  Google Scholar 

  • Heins J, Welker P, Schonlein C, Born I, Hardtrodt B, Neubert K, Tsuru D, Barth A (1988) Mechanism of proline-specific proteinases: (I) substrate specificity of dipeptidyl peptidase IV from pig kidney and proline-specific endopeptidase from Flavobacterium meningosepticurn. Biochim Biophys Acta 954:161–169

    Article  CAS  PubMed  Google Scholar 

  • Jeong BR, Fujiwara K, Kozai T (1995) Environmental control and photoautotrophic micropropagation. Hort Rev 17:826–873

    Google Scholar 

  • Jones HG (1992) Plant and microclimate: a quantitative approach to environmental plant physiology. Cambridge University Press, Cambridge, p 456

    Google Scholar 

  • Kodym A, Zapata-Arias FJ (1999) Natural light as an alternative light source for the in vitro culture of banana (Musa acuminata cv. ‗’Grande Naine’). Plant Cell Tiss Org Cult 55:141–145

    Article  Google Scholar 

  • Kozai T (1991) Photoautotrophic micropropagation. In Vitro Cell Dev Biol - Plant 27:47–51

    Article  Google Scholar 

  • Kozai T, Kubota C, Jeong BR (1997) Environmental control for the large-scale production of plants through in vitro techniques. Plant Cell Tiss Org Cult 51:49–56

  • Kozai T, Kushihashi S, Kubota C, Fujiwara K (1992) Effect of the difference between photoperiod and dark period temperatures, and photosynthetic photon flux density on the shoot length and growth of potato plantlets in vitro. J Jpn Soc Hort Sci 61:93–98

  • Kozai T, Smith MAL (1995) Environmental control in plant tissue culture — general introduction and overview. In: Aitken-Christie J, Kozai T, Smith MAL (eds) Automation and environmental control in plant tissue culture. Springer, Dordrecht, pp 301–318

  • Kuhn BC, Claudino LO, Kuhn SB, Gutierre MAMG, Mangolin CA, Machado MFPSM (2014) Micropropagation of Cattleya forbesii Lindley (Orchidaceae) using combinations of auxin and cytokinin. Pleiade 8:83–82

    Google Scholar 

  • Lambers H, Stuart Chapin III F, Pons TL (2008) Plant physiological ecology. Dordrecht, Springer, p 604

  • Li C-X, Xu Z-G, Dong R-Q, Chang S-X, Wang L-Z, Khalil-Ur-Rehman M, Tao J-M (2017) An RNA-Seq analysis of grape plantlets grown in vitro reveals different responses to blue, green, red led light, and white fluorescent light. Front Plant Sci 8:78

  • Li T, Yang Q (2015) Advantages of diffuse light for horticultural production and perspectives for further research. Front Plant Sci 6:1–6

    Article  Google Scholar 

  • Loveys BR, Scheurwater I, Pons TL, Fitter AH, Atkin OK (2002) Growth temperature influences the underlying components of relative growth rate: an investigation using inherently fast- and slow-growing plant species. Plant Cell Environ 25:975–988

    Article  Google Scholar 

  • Mano, J. (2002). Early events in environmental stresses in plants induction mechanisms of oxidative stress. In: Inze D, Montganu MV (eds) Oxidative Stress in Plants. Taylor & Francis, pp 217–246

  • Matthys D, Gielis J, Debergh P (1995) Ethylene. In: Aitken-Christi J, Kozai M, Simith LMA (eds) Automation and environmental control in plant tissue culture. Springer, Dordrecht, pp 473–491

    Chapter  Google Scholar 

  • Maxwell K, Johnson G N (2000) Chlorophyll fluorescence—a practical guide. J Exp Bot 51:659–668

  • Mazzanatti T, Calzavara AK, Pimenta JA, Oliveira HC, Stolf-Moreira R, Bianchini E (2016) Light acclimation in nursery: morphoanatomy and ecophysiology of seedlings of three light-demanding neotropical tree species. Braz J Bot 39:19–28

    Article  Google Scholar 

  • Moe R, Heins RD, Erwin J (1991) Stem elongation and flowering of the long-day plant Campanula isophylla ‘Moretti’ in response to day and night temperature alternations and light quality. Sci Hort 48:141–151

    Article  Google Scholar 

  • Mothé GPB, Netto AT, Crespo LEC, Campostrini E (2008) Photochemical efficiency and growth characteristics of sugarcane (Saccharum officinarum L.) grown in vitro at different concentrations of sucrose and light quality. Plant Cell Cult Microprop 4:84–91

    Google Scholar 

  • Nguyen QT, Kozai T (2005) Photoautotrophic micropropagation of woody species. In: Kozai T, Afreen F, Zobayed SMA (eds) Photoautotrophic (sugar-free medium) micropropagation as a new micropropagation and transplant production system. Springer, Dordrecht, pp 123–146

    Chapter  Google Scholar 

  • Otroshy M, Zamani A, Ebrahimi M, Struik PC (2009) Effect of exogenous hormones and chilling on dormancy breaking of seeds of Asafoetida (Ferula assafoetida L.). Res J Seed Sci 2:9–15

    Article  Google Scholar 

  • Pedmale UV, Huang SC, Zander M, Cole BJ, Hetzel J, Ljung K, Chory J (2016) Cryptochromes interact directly with PIFs to control plant growth in limiting blue light. Cell 164:233–245

    Article  CAS  PubMed  Google Scholar 

  • Perez Martinez LV, Melgarejo LM (2015) Photosynthetic performance and leaf water potential of gulupa (Passiflora edulis sims, passifloraceae) in the reproductive phase in three locations in the colombian andes. Acta Biolo Colomb 20:183–194

    Article  Google Scholar 

  • Pospisilová J, Solarová, Catsky J (1992) Photosynthetic responses to stress during in vitro cultivation. Photosynthetica 26:3–18

    Google Scholar 

  • Preece JE, Sutter E (1991) Acclimatization of microprop Mi cropropagation: technology and applicationagated plants to the greenhouse and field. In: Debergh PC, Zimmerman (eds) . Kluwer Academic Publishers, Dordrecht, pp 71–94

    Google Scholar 

  • Rezende RKS, Paiva LV, Paiva R, Chalfun Júnior A, Torga PP, Castro EM (2008) Organogenesis in floral chapters and evaluation of anatomical characteristics of the leaf of Gerbera jamesonni Adlam. Sci Agrotec 32:821–827

    Google Scholar 

  • SAEG (2007) System for Statistical Analysis, Version 9.1 Arthur Bernardes Foundation - Federal University of Viçosa, Viçosa – MG.

  • Schmildt O, Torres-Netto A, Schmildt ER, Carvalho VS, Otoni WC, Campostrini E (2015) Photosynthetic capacity, growth, and water relations in Golden papaya cultivated in vitro with modifications in light quality, sucrose concentration and ventilation. Theor Exp Plant Physiol 27:7–18

    Article  Google Scholar 

  • Solarova J, Souckova D, Ullmann J, Pospisilova J (1996) In vitro culture: environmental conditions and plantlet growth as affected by vessel and stopper types. Hort Sci 23:51–58

    Google Scholar 

  • Stancato GC, Bemelmans PF, Vegro CCLR (2001) Production of orchid seedlings from in vitro seeds and their economic viability: a case study. Ornam Hort 7:25–33

  • Stancato GC, Tucci MLSA (2010) Monitoring the end of the in vitro phase of Anthurium andreanum Lindl. Plantlets. Brazilian J Plant Physiol 22:61–68

    Article  Google Scholar 

  • Strasser BJ, Strasser RJ (1995) Measuring fast fluorescence transients to address environmental questions: the JIP test. In: Mathis P (ed) Photosynthesis: From Light to Biosphere. Kluwer Academic Publishers, the Netherlands, pp 977–980

    Google Scholar 

  • Strasser RJ, Srivastava A, Tsimilli-Michael M (2004) Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou G, Govindjee (eds) Advances in Photosynthesis and Respiration. Chlorophyll fluorescence: a Signature of photosynthesis. Kluwer Academic Publishers, the Netherlands, pp 321–362

  • Strasser RJ, Tsimilli-Michael M, Srivastava A (2000) The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Yunus M, Pather U, Mohanly P (eds) Probing Photosynthesis: Mechanisms. Regulation and Adaptation. Taylor and Francis, London, pp 445–483

    Google Scholar 

  • Watanabe Y, Yamaguchi M, Sakamoto J, Tamai Y (1993) Characterization of plasma membrane H+-ATPase from salt-tolerant yeast Candida versatilis. Yeast 9:213–220

    Article  CAS  PubMed  Google Scholar 

  • Wellburn AR (1994) The spectral determination of chlorophyll a and chlorophyll b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol 144(3):307–313

    Article  CAS  Google Scholar 

  • Witkowski ETF, Lamont BB (1991) Leaf specific mass confounds leaf density and thickness. Oecologia 88:486–493

    Article  CAS  PubMed  Google Scholar 

  • Xiao Y, Niu G, Kozai T (2011) Development and application of photoautotrophic micropropagation plant system. Plant Cell Tiss Org Cult 105:149–158

    Article  CAS  Google Scholar 

  • Zhang S, Ma K, Chen L (2003) Response of photosynthetic plasticity of Paeonia suffruticosa to changed light environments. Environ Exp Bot 49:121–133

    Article  Google Scholar 

  • Ziv M (1995) In vitro acclimatization. In: Aitken-Christie J, Kozai T, Smith MAL (eds) Automation and environmental control in plant tissue culture. Springer, Dordrecht, pp 493–516

    Chapter  Google Scholar 

  • Ziv M, Schwartz A, Fleminger D (1987) Malfunctioning stomata in vitreous leaves of carnation (Sianthus caryopphyllus) plants propagated in vitro, implications for hardening. Plant Sci 52:127–134

    Article  CAS  Google Scholar 

  • Zobayed S (2006) Aeration in plant tissue culture. In: Dutta Gupta S, Ibaraki Y (eds) Plant tissue culture engineering. Springer, Dordrecht, pp 313–327

    Google Scholar 

  • Zobayed SMA, Afreen F, Kozai T (2001) Physiology of Eucalyptus plantlets grown photoautotrophically in a scaled-up vessel. In Vitro Cell Dev Biol - Plant 37:807–813

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Agroecologia, Universidade Estadual do Maranhão, Av. Lourenço Vieira da Silva, s/n°, Cidade Universitária Paulo VI, Tirirical, São Luís, MA, CEP 65054-970, Brazil

    Luciene Souza Ferreira

  2. Laboratório de Fitotecnia, LFIT, CCTA, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, Campos dos Goytacazes, RJ, 28013-602, Brazil

    Andressa Leal Generoso, Virginia Silva Carvalho & Rafael Walter

  3. Departamento de Zootecnia, Universidade Estadual do Maranhão, São Luis, MA, 65055-190, Brazil

    Fábio Afonso Mazzei Moura de Assis Figueiredo & Tiago Massi Ferraz

  4. Setor de Fisiologia Vegetal, LMGV, CCTA, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, Campos dos Goytacazes, RJ, 28013-602, Brazil

    Eliemar Campostrini

  5. Citrus Nutrition and Physiology Laboratory, Centro de Citricultura Sylvio Moreira (CCSM), Instituto Agronômico (IAC), Rod. Anhanguera, km 158, CP 04, Cordeirópolis, SP, 13490-970, Brazil

    Jefferson Rangel da Silva

  6. Laboratorio de Engenharia Agricola, LEAG, CCTA, Universidade Estadual do Norte Fluminense, Av. Alberto Lamego, 2000, Campos dos Goytacazes, RJ, 28013-602, Brazil

    Geraldo de Amaral Gravina

  7. Centro de Ciências Agrárias, Naturais e Letras., Universidade Estadual da Região Tocantina do Maranhão, Avenida Brejo do Pinto, S/N, Imperatriz, Maranhão, 65975-000, Brazil

    Weverton Pereira Rodrigues

  8. Environmental Horticulture Department, University of Florida, 2043, IFAS Research Dr, Gainesville, FL, 32611-2067, USA

    Wagner A. Vendrame

Authors
  1. Luciene Souza Ferreira
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Andressa Leal Generoso
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Virginia Silva Carvalho
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fábio Afonso Mazzei Moura de Assis Figueiredo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Rafael Walter
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Tiago Massi Ferraz
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Jefferson Rangel da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Geraldo de Amaral Gravina
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Weverton Pereira Rodrigues
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Wagner A. Vendrame
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Eliemar Campostrini
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Eliemar Campostrini.

Ethics declarations

Conflict of interest

The authors declare no competing interests.

Additional information

Editor: Baochun Li

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ferreira, L.S., Generoso, A.L., Carvalho, V.S. et al. Better light spectral quality and thermal amplitude inside the greenhouse stimulate growth and improve acclimatization of in vitro–grown Cattleya warneri T. Moore. In Vitro Cell.Dev.Biol.-Plant 57, 883–896 (2021). https://doi.org/10.1007/s11627-021-10162-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-021-10162-8

Keywords

Search

Navigation