Methodological approaches to the analysis of biologically active compounds in rhizomes and roots of Eleutherococcus senticosus (Rupr. et Maxim.) Maxim. [syn. Acanthopanax senticosus (Rupr. et Maxim.) Harms] were substantiated. An HPLC method for assay of eleutheroside B (syringin) and a spectrophotometric method for determination of the total content of phenylpropanoids in terms of eleutheroside B (syringin) isolated from medicinal plant raw material after preliminary purification and filtration through a layer of aluminum oxide were developed and validated. The total content of phenylpropanoids in roots and rhizomes of E. senticosus was found to vary from 0.30 ± 0.02% to 0.37 ± 0.02%; eleutheroside B, from 0.041 ± 0.002% to 0.089 ± 0.003%. Results of statistical processing indicated that the relative error of determining the mean total of phenylpropanoids and eleutheroside B (syringin) did not exceed ±5.0% at a confidence level of 95%.
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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 56, No. 3, pp. 34 – 41, March, 2022.
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Kurkin, V.A., Ryazanova, T.K. Methodological Approaches to Standardization of Rhizomes and Roots of Eleutherococcus senticosus. Pharm Chem J 56, 366–373 (2022). https://doi.org/10.1007/s11094-022-02653-8
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DOI: https://doi.org/10.1007/s11094-022-02653-8