Abstract
A new method for targeting lung infections is of great interest using biodegradable nanoparticles. In this study, bergenin-loaded BSA NPs were developed against lung injury. Briefly, bergenin-loaded bovine serum albumin nanoparticles (BG@BSA NPs) were synthesized and characterized. HPLC recorded the major peak of bergenin. UV–Vis spectra had an absorbance at 376 nm. XRD revealed the presence of crystalline particles. FTIR confirmed the occurrence of functionalized molecules in the synthesized NPs. The particles were highly stable with a net negative charge of − 24.2. The morphology of NPs was determined by SEM and TEM. The mean particle size was 124.26 nm. The production of NO by NR8383 cells was decreased by BG@BSA NPs. Also, in mice, lipopolysaccharide-mediated acute lung inflammation was induced. BG@BSA NPs reduced macrophages and neutrophils in BALF and remarkably enhanced wet weight-to-dry weight (W/D) ratios and myeloperoxidase (MPO) activity. Further, BG@BSA NPs inhibited the production of inflammatory cells as well as tumor necrosis factor. The histopathological studies revealed that the damage and neutrophil infiltration were greatly inhibited by BG@BSA NPs. This indicates that BG@BSA NPs may be used to treat lung infections. Therefore, this study has given new insight into producing an active drug for the treatment of lung-associated diseases in the future.
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References
Rubenfeld, G.D., and M.S. Herridge. 2007. Epidemiology and outcomes of acute lung injury. Chest 131: 554–559.
Matthay, M.A. 2008. Treatment of acute lung injury: Clinical and experimental studies. Proceedings of the American Thoracic Society 5: 297–301.
Girard, T.D., and G.B. Bernard. 2007. Mechanical ventilation in ARDS: A state-of-the-art review. Chest 131: 921–933.
Sadikot, R.T., J.W. Christman, and T.S. Blackwell. 2004. Molecular targets for modulating lung inflammation and injury. Current Drug Targets 5: 581–588.
Yuan, Z., M. Syed, D. Panchal, M. Joo, C. Bedi, S. Lim, H. Onyuksel, I. Rubinstein, M. Colonna, and R.T. Sadikot. 2016. TREM-1-accentuated lung injury via miR-155 is inhibited by LP17 nanomedicine. American Journal of Physiology. Lung Cellular and Molecular Physiology 310: L426–L438.
Sadikot, R.T., and I. Rubinstein. 2009. Long-acting, multi-targeted nanomedicine: Addressing unmet medical need in acute lung injury. Journal of Biomedical Nanotechnology 5: 614–619.
Foldvari, M., and N. Elsabahy. 2011. Nanotechnology enables superior medical therapies. Current Drug Delivery 8: 225–226.
Dames, P., B. Gleich, A. Flemmer, K. Hajek, N. Seidl, F. Wiekhorst, D. Eberbeck D, I. Bittmann, C. Bergemann, T. Weyh, L. Trahms, and J. Rosenecker. 2007. Targeted delivery of magnetic aerosol droplets to the lung. Nature Nanotechnology. 2(8):495–499.
Kim, B.Y., J.T. Rutka, and W.C. Chan. 2010. Nanomedicine. New England Journal of Medicine 363: 2434–2443.
Singh, S. 2010. Nanomedicine-nanoscale drugs and delivery systems. Journal of Nanoscience and Nanotechnology 10: 7906–7918.
Buxton, D.B. 2009. Nanomedicine for the management of lung and blood diseases. Nanomedicine 4: 331–339.
Roy, I., and N. Vij. 2010. Nanodelivery in airway diseases: Challenges and therapeutic applications. Nanomedicine 6: 237–244.
Klingler, C., B.W. Müller, and H. Steckel. 2009. Insulin-micro- and nanoparticles for pulmonary delivery. International Journal of Pharmaceutics 377 (377): 173–179.
Shuvaev, V.V., and V.R. Muzykantov. 2011. Targeted modulation of reactive oxygen species in the vascular endothelium. Journal of Controlled Release 153: 56–63.
Sung, J.C., B.L. Pulliam, and D.A. Edwards. 2007. Nanoparticles for drug delivery to the lungs. Trends in Biotechnology 25: 563–570.
Schleh, C., B. Rothen-Rutishauser, and W.G. Kreyling. 2011. The influence of pulmonary surfactant on nanoparticulate drug delivery systems. European Journal of Pharmaceutics and Biopharmaceutics 770: 350–352.
Weber, C., J. Kreuter, and K. Langer. 2000. Desolvation process and surface characteristics of HAS-nanoparticles. International Journal of Pharmaceutics 196: 197–200.
Bhushan, B., P. Dubey, S. Uday Kumar, A. Sachdev, I. Mataia, and P. Gopinath. 2015. Bionanotherapeutics: niclosamide encapsulated albumin nanoparticles as a novel drug delivery system for cancer therapy. RSC Advances 5:12078.
Hossain, M., A. Y. Khan, G. S. Kumar. 2011. Interaction of the anticancer plant alkaloid sanguinarine with bovine serum albumin. PLoS One 6: e18333.
Maltas, E. 2014. Binding interactions of niclosamide with serum proteins. Journal of Food and Drug Analysis 22: 549–555.
Abdelwahed, W., G. Degobert, S. Stainmesse, and H. Fessi. 2006. Freeze-drying of nanoparticles: Formulation, process and storage considerations. Adv. Drug Delivery Rev. 58: 1688–1713.
Peer, D., J.M. Karp, S. Hong, O.C. Farokhzad, R. Margalit, and R. Langer. 2007. Nanocarriers as an emerging platform for cancer therapy. Nature Nanotechnology 2: 751–760.
Guo, H., S. Fei, Y. Zhang, Y.u. Zhang, J. Gou, L. Zhang, H. He, T. Yin, Y. Wang, and X. Tang. 2016. Teniposide-loaded multilayer modified albumin nanoparticles with increased passive delivery to the lung. RSC Advances 6: 81110.
Sripriyalakshmi, S., C. H. Anjali, C. G. P. Doss, B. Rajith, and A. Ravindran. 2014. BSA nanoparticle loaded atorvastatin calcium--a new facet for an old drug. PLoS One 9: e86317.
Wang, Y., W. Huang, J. Zhang, M. Yang, Q. Qi, K. Wang, A. Lia, and Z. Zhao. 2016. The therapeutic effect of Bletilla striata extracts on LPS-induced acute lung injury by regulation of inflammation and oxidation. RSC Advances 6: 89338.
Nanashima, N., M. Akita, T. Yamada, T. Shimizu, H. Nakano, Y. Fan, and S. Tsuchida. 2008. The hairless phenotype of the Hirosaki hairless rat is due to the deletion of an 80-kb genomic DNA containing five basic keratin genes. Journal of Biological Chemistry 283: 16868–16875.
Ma, C., J. Liu, R. Qu, S. Ma, and Chin. 2014. Tectorigenin inhibits the inflammation of LPS-induced acute lung injury in mice. Chinese Journal of Natural Medicines 12:841–846.
Huang, X., Y. Liu, Y. Lu, and C. Ma. 2015. Anti-inflammatory effects of eugenol on lipopolysaccharide-induced inflammatory reaction in acute lung injury via regulating inflammation and redox status. International Immunopharmacology 26: 265–271.
Türüt, H., H. Ciralik, M. Kilinc, D. Ozbag, and S.S. Imrek. 2009. Effects of early administration of dexamethasone, N-acetylcysteine and aprotinin on inflammatory and oxidant-antioxidant status after lung contusion in rats. Injury 40: 521–527.
Ohkawa, H., N. Ohishi, and K. Yagi. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry 95: 351–358.
Koul, A., V. Bhatia, and M.P. Bansal. 2001. Effect of alpha-tocopherol on pulmonary antioxidant defence system and lipid peroxidation in cigarette smoke inhaling mice. BMC Biochemistry 2: 1471–2091.
Jiang, W., F. Luo, Q. Lu, J. Liu, P. Li, X. Wang, Y. Fu, K. Hao, T. Yan, and X. Ding. 2016. Chemico-Biological Interactions 243: 127–134.
Ma, J., H. Xu, J. Wu, C. Qu, F. Sun, and S. Xu. 2015. Linalool inhibits cigarette smoke-induced lung inflammation by inhibiting NF-κB activation. International Immunopharmacology 29: 708–713.
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Lingqiao Yan contributed to the conception and coordination of the study, the assessment of the quality of trials, and drafting the manuscript. Hui Chen contributed to the extracting and screening of all references for eligibility, the assessment of the quality of trials, and drafting the manuscript. Mindan Xie contributed to the conception and coordination of the study, the assessment of the quality of trials, and drafting the manuscript.
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In this study, all animal procedures were performed in accordance with the Guidelines for Care and Use of Laboratory Animals of First People’s Hospital of Wenling and all experiments were conducted with the approval of the Animal Ethical Committee at the First People’s Hospital of Wenling, People’s Republic of China.
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Hui Lin, Pengfei Wang and Wanhong Zhang contributed equally to this study
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Lin, H., Wang, P., Zhang, W. et al. Novel Combined Preparation and Investigation of Bergenin-Loaded Albumin Nanoparticles for the Treatment of Acute Lung Injury: In Vitro and In Vivo Evaluations. Inflammation 45, 428–444 (2022). https://doi.org/10.1007/s10753-021-01556-2
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DOI: https://doi.org/10.1007/s10753-021-01556-2