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LncRNA PVT1 delays skin photoaging by sequestering miR-551b-3p to release AQP3 expression via ceRNA mechanism

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Abstract

Understanding human skin photoaging requires in-depth knowledge of the molecular and functional mechanisms. Human dermal fibroblasts (HDFs) gradually lose their ability to produce collagen and renew intercellular matrix with aging. Therefore, our study aims to reveal the mechanistic actions of a novel ceRNA network in the skin photoaging by regulating HDF activities. Photoaging-related genes were obtained in silico, followed by GO and KEGG enrichment analyses. Differentially expressed lncRNAs and miRNAs were screened from the GEO database to construct the ceRNA co-expression network. In skin photoaging samples, PVT1 and AQP3 were poorly expressed, while miR-551b-3p was highly expressed. The relationships among the lncRNA, miRNA and mRNA were explored through the ENCORI database and dual luciferase reporter assay. Mechanistically, PVT1 could sequester miR-551b-3p to upregulate the expression of AQP3, which further inactivated the ERK/p38 MAPK signaling pathway. HDFs were selected to construct an in vitro cell skin photoaging model, where the senescence, cell cycle distribution and viability of young and senescent HDFs were detected by SA-β-gal staining, flow cytometry and CCK-8 assay. In vitro cell experiments confirmed that overexpression of PVT1 or AQP3 enhanced viability of young and senescent HDFs and inhibited HDF senescence, while miR-551b-3p upregulation counteracted the effect of PVT1. In conclusion, PVT1-driven suppression of miR-551b-3p induces AQP3 expression to inactivate the ERK/p38 MAPK signaling pathway, thereby inhibiting HDF senescence and ultimately delaying the skin photoaging.

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Data Availability

The data that supports the findings of this study are available on request from the corresponding author upon reasonable request.

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Acknowledgements

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Funding

This study was supported by Project of Hunan Provincial Finance Department (No. 2021CZT04).

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Authors and Affiliations

  1. Department of Dermatology, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), No. 61, Jiefang West Road, Furong District, Changsha, 410000, Hunan Province, P. R. China

    Hua Tang, Qi Xiong, Ming Yin, Hao Feng, Fang Yao, Xiao Xiao, Feng Hu & Yangying Liao

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  1. Hua Tang
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  2. Qi Xiong
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  3. Ming Yin
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  4. Hao Feng
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  7. Feng Hu
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  8. Yangying Liao
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Contributions

Hua Tang, Qi Xiong and Ming Yin wrote the paper and conceived and designed the experiments; Hao Feng, Fang Yao and Xiao Xiao analyzed the data; Feng Hu and Yangying Liao collected and provided the sample for this study. All authors have read and approved the final submitted manuscript.

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Correspondence to Yangying Liao.

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Tang, H., Xiong, Q., Yin, M. et al. LncRNA PVT1 delays skin photoaging by sequestering miR-551b-3p to release AQP3 expression via ceRNA mechanism. Apoptosis 28, 912–924 (2023). https://doi.org/10.1007/s10495-023-01834-4

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