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A disposable edoxaban sensor chip using carbon paste electrode grafted with molecularly imprinted polymer

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Abstract

Although direct oral anticoagulants (DOACs) are generally safe and TDM is not required, blood levels of the drug are important information for response decisions in emergency care. In this study, an attempt was made to develop a disposable sensor chip for the rapid detection of edoxaban in blood, a type of DOAC. Molecularly imprinted polymers with edoxaban tosilate as a template and sodium p-styrene sulfonate as a functional monomer were grafted onto the surface of graphite particles, mixed with silicon oil dissolved in ferrocene to form a paste, and filled onto a substrate made of plastic film. Sensor chips were fabricated. The current obtained from this sensor by voltammetry within 150 s depended on the edoxaban concentration. Sensitivity to edoxaban was also confirmed in bovine whole blood. The potential of disposable sensors to rapidly detect edoxaban in whole blood was demonstrated in this study, although selectivity, reproducibility, and sensitivity need to be improved for practical use.

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The datasets generated or analyzed during the current study are available from the corresponding author on reasonable request.

References

  1. Crader MF, Johns T, Arnold JK. Warfarin drug interactions. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022

  2. Holbrook A, Schulman S, Witt DM, Vandvik PO, Fish J, Kovacs MJ, Svensson PJ, Veenstra DL, Crowther M, Guyatt GH. Evidence-based management of anticoagulant therapy: antithrombotic therapy and prevention of thrombosis, 9th ed: American College of Chest Physicians evidence-based clinical practice guidelines. Chest. 2012;141(2 Suppl):e152S-e184S.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Gosselin RC, Adcock DM, Bates SM, Douxfils J, Favaloro EJ, Gouin-Thibault I, Guillermo C, Kawai Y, Lindhoff-Last E, Kitchen S. International council for standardization in haematology (ICSH) recommendations for laboratory measurement of direct oral anticoagulants. Thromb Haemost. 2018;118:437–50. https://doi.org/10.1055/s-0038-1627480.

  4. Douxfils J, Adcock DM, Bates SM, Favaloro EJ, Gouin-Thibault I, Guillermo C, Kawai Y, Lindhoff-Last E, Kitchen S, Gosselin RC. 2021 update of the international council for standardization in haematology recommendations for laboratory measurement of direct oral anticoagulants. Thromb Haemost. 2021;121:1008–20. https://doi.org/10.1055/a-1450-8178.

  5. Hanada K, Matsumoto SI, Shibata S, Matsubara H, Tsukimura Y, Takahashi H. A quantitative LC/MSMS method for determination of edoxaban, a Xa inhibitor and its pharmacokinetic application in patients after total knee arthroplasty. Biomed Chromatogr. 2018;32:e4213. https://doi.org/10.1002/bmc.4213.

  6. Yoshimi Y, Yagisawa Y, Yamaguchi R, Seki M. Blood heparin sensor made from a paste electrode of graphite particles grafted with molecularly imprinted polymer. Sens Actuators B Chem. 2018;259:455–62. https://doi.org/10.1016/j.snb.2017.12.084.

    Article  CAS  Google Scholar 

  7. Aaryashree, Ohishi T, Yoshimi Y. A disposable sensor chip using a paste electrode with surface-imprinted graphite particles for rapid and reagentless monitoring of theophylline. Molecules. 2022. https://doi.org/10.3390/molecules27082456.

  8. Aaryashree, Takeda Y, Kanai M, Hatano A, Yoshimi Y, Kida M. A "single-use" ceramic-based electrochemical sensor chip using molecularly imprinted carbon paste electrode. Sensors (Basel). 2020. https://doi.org/10.3390/s20205847.

  9. Eguchi H, Hatano A, Yoshimi Y. Reagentless sensing of vancomycin using an indium tin oxide electrode grafted with molecularly imprinted polymer including ferrocenyl group. Sensors (Basel). 2021. https://doi.org/10.3390/s21248338.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Ruff CT, Giugliano RP, Braunwald E, Morrow DA, Murphy SA, Kuder JF, Deenadayalu N, Jarolim P, Betcher J, Shi M, Brown K, Patel I, Mercuri M, Antman EM. Association between edoxaban dose, concentration, anti-Factor Xa activity, and outcomes: an analysis of data from the randomised, double-blind ENGAGE AF-TIMI 48 trial. Lancet. 2015;385:2288–95. https://doi.org/10.1016/S0140-6736(14)61943-7.

  11. Reiffel JA. Oral anticoagulation and antiarrhythmic drug therapy for atrial fibrillation. J Innov Card Rhythm Manag. 2018;9:3446–52. https://doi.org/10.19102/icrm.2018.091201.

  12. Yoshimi Y, Oino D, Ohira H, Muguruma H, Moczko E, Piletsky SA. Size of heparin-imprinted nanoparticles reflects the matched interactions with the target molecule. Sensors (Basel). 2019;19:2415. https://doi.org/10.3390/s19102415.

  13. Yoshimi Y, Katsumata Y, Osawa N, Ogishita N, Kadoya R. Nanomaterials (Basel). Synthesis of fluorescent molecularly imprinted polymer nanoparticles sensing small neurotransmitters with high selectivity using immobilized templates with regulated surface density. Nanomaterials. 2023;13:212. https://doi.org/10.3390/nano13010212.

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Acknowledgements

This study was partially supported by research funding from Daiichi Sankyo Company, Limited, the Mera-Grant of the Japanese Society for Artificial Organs (2016), and the Kenzo Suzuki Memorial Research Grant (2017).

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Authors and Affiliations

  1. Department of Applied Chemistry, Shibaura Institute of Technology, 3-7-5 Toyosu Koto-ku, Tokyo, 135-8548, Japan

    Yasuo Yoshimi, Shohei Kani &  Aaryashree

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  1. Yasuo Yoshimi
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  2. Shohei Kani
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  3. Aaryashree
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Correspondence to Yasuo Yoshimi.

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Yoshimi, Y., Kani, S. & Aaryashree A disposable edoxaban sensor chip using carbon paste electrode grafted with molecularly imprinted polymer. J Artif Organs 27, 77–81 (2024). https://doi.org/10.1007/s10047-023-01392-1

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  • DOI: https://doi.org/10.1007/s10047-023-01392-1

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