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Effects of sodium hexametaphosphate microparticles or nanoparticles on the growth of saliva-derived microcosm biofilms

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Abstract

Objectives

This study evaluated the effects of sodium hexametaphosphate microparticles (HMPmicro) or nanoparticles (HMPnano) on the growth of saliva-derived microcosm biofilms

Materials and methods

Saliva-derived biofilms were formed on glass coverslips for 24 h. Thereafter, Streptococcus mutans (C180-2) was incorporated or not into the biofilms. From that time point onwards, solutions containing 0.2% HMPmicro or HMPnano, combined or not with 220 ppm F, were constantly present in the culture medium. In addition, 220 ppm F alone (220F) and McBain medium without any compound were also tested as positive and negative controls (CTL), respectively. After 96 h, the biofilms were plated on anaerobic blood agar or sucrose agar bacitracin for total and S. mutans CFU-counting, respectively. Biofilms’ lactic acid production was analysed spectrophotometrically. Data were submitted to ANOVA or Kruskal-Wallis’ tests, followed by Student-Newman-Keuls’ test (p<0.05; n=12).

Results

HMPmicro or HMPnano led to significantly lower lactic acid production, and significant reductions in total CFU-counting in microcosm biofilms, supplemented or not with S. mutans, in comparison to both controls, with significant differences between 220F and CTL. No significant differences were observed among the groups treated with HMPmicro or HMPnano (with or without F). The same trend was seen for S. mutans CFU-counting, in biofilms supplemented with S. mutans.

Conclusions

HMP significantly reduced total and S. mutans CFU counts, as well as lactic acid production by saliva-derived microcosm biofilms.

Clinical relevance

These findings in saliva-derived microcosm biofilms suggest that HMP stands as a promising alternative for the control of cariogenic biofilms.

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Acknowledgements

The authors acknowledge Prof. Emerson Rodrigues de Camargo and Dr. Francisco Nunes Souza Neto from the Department of Chemistry of the Federal University of São Carlos (UFSCar), São Carlos (São Paulo, Brazil), for providing sodium hexametaphosphate nanoparticles for the experiments.

Funding

This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001, and CAPES/PRINT (88887.371644/2019-00).

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Authors and Affiliations

  1. Department of Preventive and Restorative Dentistry, São Paulo State University (UNESP), School of Dentistry, Rua José Bonifácio, 1193, 16015-050, Araçatuba, São Paulo, Brazil

    Caio Sampaio, Igor Zen, Thayse Yumi Hosida, Douglas Roberto Monteiro, Alberto Carlos Botazzo Delbem & Juliano Pelim Pessan

  2. Department of Preventive Dentistry, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, The Netherlands

    Dongmei Deng & Rob Exterkate

  3. Postgraduate Program in Health Sciences, University of Western São Paulo (UNOESTE), Presidente Prudente, São Paulo, Brazil

    Douglas Roberto Monteiro

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  1. Caio Sampaio
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Correspondence to Juliano Pelim Pessan.

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Sampaio, C., Deng, D., Exterkate, R. et al. Effects of sodium hexametaphosphate microparticles or nanoparticles on the growth of saliva-derived microcosm biofilms. Clin Oral Invest 26, 5733–5740 (2022). https://doi.org/10.1007/s00784-022-04529-3

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