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Differentiation of benign and malignant regions in paraffin embedded tissue blocks of pulmonary adenocarcinoma using micro CT scanning of paraffin tissue blocks: a pilot study for method validation

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Abstract

Purpose

Micro computed tomography (micro-CT) can provide detailed information about the internal structure of materials. This study aimed to demonstrate the diagnostic value of micro-CT in formalin fixed paraffin embedded pulmonary adenocarcinomas by correlating the micro-CT findings of tumoral and non-tumoral areas with hematoxylin and eosin (HE) sections.

Methods

Paraffin blocks obtained from three adenocarcinomas were scanned with micro-CT. Ten regions of interest (ROIs) from adenocarcinoma and 11 ROIs from pulmonary parenchyma (ROI-C and ROI-N, respectively) areas were compared regarding the various structural parameters.

Results

All parameters were significantly different regarding the tumoral and non-tumoral ROIs. The percent object volume, structure thickness, structure linear density, connectivity and connectivity density were higher in ROI-Cs (p < 0.000, p < 0.000, p = 0.001, p < 0.000, and p < 0.000 respectively); whereas intersection surface and structure model index were higher in ROI-Ns (p < 0.000 and p < 0.000). The open porosity percentage was higher in ROI-Ns (68.86 + 2.96 vs 48.29 + 5.11, p < 0.000) and the closed porosity percentage was higher in ROI-Cs (2.29 + 0.55 vs 0.57 + 0.17 p < 0.000).

Conclusions

The tumoral and non-tumoral areas in paraffin blocks can be distinguished from each other, using the quantitative and qualitative information obtained by micro-CT. Making this distinction with quantitative data obtained from micro-CT can therefore be the basis of creating artificial intelligence algorithms in the future.

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Abbreviations

CT:

Computed tomography

LC:

Lung cancer

SD:

Standard deviation

Min–max:

Minimum–maximum

Micro-CT:

Micro computed tomography

3D:

Three dimensional

HE:

Hematoxylin and eosin

FFPE:

Formalin fixed and paraffin embedded

ROI:

Region of interest

ROI-C:

ROI-carcinoma

ROI-N:

ROI- non-tumoral pulmonary parenchyma

OV/TV:

Percent object volume

TV:

Tissue volume

ST:

Structural thickness

IS:

Intersection surface

SMI:

Structure model index

SLD:

Structure linear density

Cn:

Connectivity

CnD:

Connectivity density

WSI:

Whole slide imaging

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Funding

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. FEBTS, Department of Thoracic Surgery, Faculty of Medicine, Ankara University, Ankara University Medical Design Application and Research Center (MEDITAM), Sıhhiye, 06230, Ankara, Turkey

    Ayten Kayı Cangır

  2. Department of Pathology, Faculty of Medicine, Ankara University, Sıhhiye, 06230, Ankara, Turkey

    Serpil Dizbay Sak

  3. Department of Thoracic Surgery, Faculty of Medicine, Ankara University, Sıhhiye, 06230, Ankara, Turkey

    Gökalp Güneş

  4. Deparment of Dentoaxillofacial, Radiology Faculty of Dentistry, Ankara University, Ankara University Medical Design Application and Research Center (MEDITAM), Ankara, Turkey

    Kaan Orhan

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  1. Ayten Kayı Cangır
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Correspondence to Ayten Kayı Cangır.

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Kayı Cangır, A., Dizbay Sak, S., Güneş, G. et al. Differentiation of benign and malignant regions in paraffin embedded tissue blocks of pulmonary adenocarcinoma using micro CT scanning of paraffin tissue blocks: a pilot study for method validation. Surg Today 51, 1594–1601 (2021). https://doi.org/10.1007/s00595-021-02252-2

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  • DOI: https://doi.org/10.1007/s00595-021-02252-2

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