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Ameliorative effect of mussel-derived ACE inhibitory peptides on spontaneous hypertension rats

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Abstract

Purpose

The purpose of this study was to prepare the novel mussel-derived ACE inhibitory peptides (MEPs) by enzymatic hydrolysis of Mytilus edulis and investigate their antihypertensive effects in vivo.

Methods

After assessing the stability of MEPs in vitro, we investigated the effect of MEPs on hypertension using spontaneously hypertensive rats (SHRs). Subsequently, MEPs were purified and identified by ultrafiltration, gel filtration chromatography and liquid chromatography-tandem mass spectrometry (LC–MS/MS).

Results

Our study demonstrated that MEPs could keep stable ACE inhibitory activity after treatment with heat, acid, alkali, metal ions and simulated gastrointestinal digestive fluid. Additionally, the animal experiments showed that both short-term and long-term treatment with MEPs resulted in a significant reduction in systolic and diastolic blood pressure in SHRs. Mechanistically, the results suggested that MEPs could reduce vascular remodeling, regulate renin-angiotensin system (RAS), and inhibit kidney and myocardial fibrosis. Finally, we isolated and identified five peptides from MEPs, with the peptide Ile-Leu-Thr-Glu-Arg showed the highest ACE inhibition rate.

Conclusion

Our findings demonstrate the potential use of MEPs as active components in functional foods designed to lower blood pressure.

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Date availability

The datasets generated during and analyzed during the current study are available from the corresponding author upon reasonable request.

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Acknowledgements

This study was financially supported by Qingdao key technology research and industrialization demonstration projects (22-3-3-hygg-5-hy).

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Authors and Affiliations

  1. College of Life Sciences, Qingdao University, 308 Ningxia Road, Qingdao, 266071, China

    Qiaoni You, Xiaopeng Sun, Jia Yu & Yuxi Wei

  2. Chenland Nutritionals, Incorporated, Invine, CA, USA

    Jinli Chen

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  1. Qiaoni You
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  2. Xiaopeng Sun
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  4. Jia Yu
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Contributions

Investigation, methodology, data analysis, validation, writing-original draft, QY; Methodology, data analysis, XS; Resources, validation, JC; Supervision, writing—review and editing, JY; Conceptualization, supervision, funding acquisition, writing—review and editing, YW. All authors: read and approved the final manuscript.

Corresponding authors

Correspondence to Jia Yu or Yuxi Wei.

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Conflict of interest

There are no conflicts of interest in this paper.

Ethical approval

This study was permitted by the Qingdao University Animal Protection and Use Ethics Committee (Animal Ethics Certificate NO.20210917SHR241020030) and the experimental procedures strictly conducted in compliance with the International Animal Protection Directive.

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The manuscript does not contain clinical studies or patient data.

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You, Q., Sun, X., Chen, J. et al. Ameliorative effect of mussel-derived ACE inhibitory peptides on spontaneous hypertension rats. Eur J Nutr 62, 3097–3111 (2023). https://doi.org/10.1007/s00394-023-03222-9

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