Abstract
Sleep is conserved in the animal kingdom and plays a pivotal role in the adaptation of species. Sleep in Drosophila melanogaster is defined as any continuous 5 min of quiescence, shows a prominent siesta, and consolidated nighttime sleep. Here, we analyzed the sleep of two other species D. malerkotliana (DMK) and D. ananassae (DA), and compared it with D. melanogaster (DM). The DMK males and females have siesta like DM. However, unlike DM, flies continue to sleep beyond siesta till the evening. DA has a less prominent siesta compared to DM and DMK. In the morning, DA took a longer time to respond to the lights ON and continued to sleep for at least half an hour. The nighttime sleep of the DA flies is higher than the other two species. Average length of sleep episode is three times more than that of DM and DMK with few wake episodes. Thus, the nighttime sleep of DA males and females is deep and needs exposure to more potent stimuli to wake up relative to the other two species. DA males and females show higher sleep rebound than the other two species, suggesting the robustness of sleep homeostasis. Although total sleep of DMK and DA is similar, DA is a day-active species with highly consolidated night sleep. DMK, like DM, is a crepuscular species with a midday siesta. Thus, our results suggest that temporal partitioning of sleep, in sympatric species may contribute to temporal segregation.
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Acknowledgements
We thank Sheeba Vasu for fly lines. We thank Science and Engineering Research board for Ramanujan fellowship (SB/S2/RJN-005/2016) and EMR/2017/001625 to SRL. We will also like to acknowledge CSIR for fellowship to Nisha Sharma and Sukriti Mishra. We thank Sheetal Potdar for reading our manuscript and some useful suggestions. We also thank three anonymous reviewers for useful suggestions.
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Mishra, S., Sharma, N., Singh, S.K. et al. Peculiar sleep features in sympatric species may contribute to the temporal segregation. J Comp Physiol B 193, 57–70 (2023). https://doi.org/10.1007/s00360-022-01463-4
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DOI: https://doi.org/10.1007/s00360-022-01463-4