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A comparative study on the influences of platelet-rich plasma vs its derived cytokines on skin rejuvenation

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Abstract

Background

Platelet-rich plasma (PRP), a cytokine therapy, concentrates the growth factors in platelets and is considered independent of platelet function. We compared the effectiveness of cell-free PRP cytokine (PRP-C) and ordinary PRP therapies on skin rejuvenation.

Methods

We selected 1340 patients (125 men and 1215 women) who received facial treatment from February 2020 to June 2022. PRP (n = 705) or PRP-C (n = 635) therapies were chosen by the patient; 6 ml of the substance was administered intradermally or subcutaneously. PRP was prepared from peripheral blood using sodium citrate solution and centrifugation and was used directly, or further frozen and centrifuged and the lysate was used as PRP-C.

Results

Patient satisfaction improved significantly after 4 and 8 weeks of treatment in both the PRP and PRP-C therapy groups (p < 0.0001 for all four values). No significant difference was reported in the therapeutic effect between the two treatment groups after 4 and 8 weeks (p = 0.119 and p = 0.6686, respectively).

Doctor evaluation efficacy of both therapies improved at both time points (p < 0.0001 for all four values). However, at 4 weeks, doctors estimated a more significant improvement in the PRP therapy group than in the PRP-C group; 8 weeks later, no significant differences were observed.

Conclusions

Both therapies improved skin texture and elasticity. The efficacy rate was greater than the improvement for wrinkles. The effectiveness of nasolabial lines and facial lift was not significant. PRP-C therapy can be used as cell-free therapy.

Level of evidence: level III, therapeutic study

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Funding

The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Ginza Yoshie Clinic, 1-23-14 Hiramachi, Meguro, Tokyo, 152-0032, Japan

    Yoshie Hirose, Akira Aoki & Hajime Inoue

  2. Laboratory of Cell Applied Technologies Co., 1-23-14 Hiramachi, Meguro, Tokyo, 152-0032, Japan

    Chiharu Fujita

  3. Plastic & Reconstructive Surgery, St. Marianna University School of Medicine, 2-16-1 Sugao, Miyamae, Kawasaki, 216-8511, Japan

    Hajime Inoue

Authors
  1. Yoshie Hirose
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  2. Chiharu Fujita
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  3. Akira Aoki
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  4. Hajime Inoue
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Corresponding author

Correspondence to Hajime Inoue.

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Ethics approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. This study was reviewed by The Technical Advisor Group of the Extraordinary Certified Committee for Regenerative Medicine of Yukeikai (Ref. No. NA8200002) recognized by the Safety of Regenerative Medicine Act regulated by the Ministry of Health, Labour, and Welfare based on, and then approved (Approval Nos. PC3200287, PC3200288, PC3200289, PC3200290, PC3200271, PC3210061, PC3210213).

Consent to participate

Informed consent was obtained from all patients for the inclusion of their data (clinical and patient-reported outcomes) for the purpose of publication.

Consent for publication

The authors affirm that the human research participants provided informed consent for the publication of the images in Figs. 1, 2, and 3.

Competing interests

Hirose Yoshie, Fujita Chiharu, Aoki Akira, and Inoue Hajime declare no competing interests.

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Hirose, Y., Fujita, C., Aoki, A. et al. A comparative study on the influences of platelet-rich plasma vs its derived cytokines on skin rejuvenation. Eur J Plast Surg 46, 725–732 (2023). https://doi.org/10.1007/s00238-023-02063-3

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  • DOI: https://doi.org/10.1007/s00238-023-02063-3

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