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Periosteal Bone Formation Varies with Age in Periostin Null Mice

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Abstract

Periostin, also known as osteoblast-specific factor 2, is a matricellular protein predominantly expressed at the periosteum of bone. During growth and development, periostin contributes to periosteal expansion by facilitating osteoblast differentiation and mineralization. Later in life, periosteal expansion provides an adaptive strategy to increase tissue strength without requiring substantial increase in bone mass. However, the function of periostin past skeletal maturity and during advanced aging is relatively unknown. The objective of this study was to examine the function of periostin in maintaining bone mass and tissue strength across different ages. In periostin null mice (Postn−/−), periosteal bone formation was significantly reduced in young (3 months) and adult mice (9 months). The lack of bone formation resulted in reduced bone mass and ultimate strength. Conversely, periosteal bone formation increased at advanced ages in 18-month-old Postn−/− mice. The increase in periosteal mineralization at advanced ages coincides with increased expression of vitronectin and osteopontin. Periosteal progenitors from Postn−/− mice displayed an increased capacity to mineralize when cultured on vitronectin, but not type-1 collagen. Altogether, these findings demonstrate the unique role of periostin in regulating periosteal bone formation at different ages and the potential for vitronectin to compensate in the absence of periostin.

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Acknowledgements

This study was supported by NIH/NIAMS Grant R01AR076378.

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Authors and Affiliations

  1. Department of Orthopedics, Bone and Joint Center, Henry Ford Health System, 6135 Woodward Avenue, Detroit, MI, 48202, USA

    Joseph D. Gardinier, Amit Chougule & Chunbin Zhang

  2. School of Medicine, Wayne State University, Detroit, MI, USA

    Devin Mendez

  3. Department of Orthopaedic Surgery, University of Michigan, Ann Arbor, MI, USA

    Conor Daly-Seiler

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  1. Joseph D. Gardinier
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  2. Amit Chougule
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  3. Devin Mendez
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  4. Conor Daly-Seiler
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  5. Chunbin Zhang
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Correspondence to Joseph D. Gardinier.

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Disclosure

Joseph Gardinier, Amit Chougule, Devin Mendez, Conor Daly-Seiler, and Chunbin Zhang declare that they have no conflict of interest.

Human and Animal Rights and Informed Consent

Animal procedures were conducted under Institutional Animal Care Use Committee (IACUC) approval at the Henry Ford Hospital. The ethical principles established by the National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH Publications No. 8523, revised 2011) were followed. The study does not involve research in humans.

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Gardinier, J.D., Chougule, A., Mendez, D. et al. Periosteal Bone Formation Varies with Age in Periostin Null Mice. Calcif Tissue Int 112, 463–471 (2023). https://doi.org/10.1007/s00223-023-01063-6

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  • DOI: https://doi.org/10.1007/s00223-023-01063-6

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